BoP course 1997

BoP bioinformatics module

July 14-15, 2018
Instructors: Omar Harb and Dan Beiting

• Exercises:
• RNA sequence data analysis via Galaxy, Part I Uploading data and starting the workflow
• RNA sequence data analysis Part II: viewing and analyzing your results
• Identifying SNPs using Galaxy, Part I
• Identifying SNPs using Galaxy, Part II: Viewing and analyzing your results
• Genome Browser II: Interpreting RNAseq data
• High resolution image
  
• Useful resource:
• FastQC output
• DeSeq2 Beginner's guide
• SnpEff Documentation
• Trimmomatic Manual
• RNA-seq differential expression studies: more sequence or more replication?
• Gaining comprehensive biological insight into the transcriptome by performing a broad-spectrum RNA-seq analysis
• Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2
• DIY Transcriptomics
  
• Lectures:
• Lecture 1 - RNAseq intro/FAQs
• Lecture 2 - dealing with count data